plin5 recombinant peptide  (Novus Biologicals)

Journal: The Journal of Physiology

Article Title: Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5

doi: 10.1113/jphysiol.2012.240952

Figure Lengend Snippet: Representative immunofluorescence images of PLIN5 (A), IMTG (B), and the merged images (C) with the yellow areas describing regions of overlap between PLIN5 and IMTG images. The yellow objects were then extracted (to measure the number of PLIN5-LDs; D), and the number subtracted from the total number of LDs to quantify the number of PLIN5-null-LD. The same procedure was used to obtain values of colocalization between PLIN2 and IMTG. White bar, 5 μm. The number of PLIN2-LD (filled bars) and PLIN2-null-LD (open bars) were quantified before (PreEx) and after exercise (PostEx), prior to (E) and following (F) training in both the SIT and ET groups. The same analysis was repeated for PLIN5 for pre- (G) and post-training (H). *Main effect of exercise bout (P < 0.05 vs. pre-exercise).

Article Snippet: The specificity of the PLIN5 antibody was confirmed in a competition experiment in which a PLIN5 recombinant peptide (Cat. no. NB110–60511PEP; Novus Biologicals, Cambridge, UK) was incubated with the PLIN5 primary antibody, subsequently resulting in removal of the positive fluorescent signal for PLIN5 (Supplementary Fig. S1 ).

Techniques: Immunofluorescence

Journal: The Journal of Physiology

Article Title: Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5

doi: 10.1113/jphysiol.2012.240952

Figure Lengend Snippet: Representative immunofluorescence images of PLIN5 (stained green) in combination with WGA to identify the cell border (stained blue) in skeletal muscle, pre- (A) and post- (B) 6 weeks of SIT. Type I fibres are indicated with a ‘I’, all other fibres are assumed type II fibres. White bar, 50 μm. PLIN5 expression, quantified from immunofluorescence images of PLIN5, in type I and type II fibres obtained before (C) and after (D) 6 weeks of SIT (filled bars) or ET (open bars). PLIN5 expression quantified from immunofluorescence images correlated with PLIN5 expression determined following immunoblotting of whole muscle homogenates (E). Values are presented as means ± SEM (n= 8 per group). *Main effect of fibre type (P < 0.05 vs. type I fibres). †Main effect of training intervention (P < 0.05 vs. pre-training).

Article Snippet: The specificity of the PLIN5 antibody was confirmed in a competition experiment in which a PLIN5 recombinant peptide (Cat. no. NB110–60511PEP; Novus Biologicals, Cambridge, UK) was incubated with the PLIN5 primary antibody, subsequently resulting in removal of the positive fluorescent signal for PLIN5 (Supplementary Fig. S1 ).

Techniques: Immunofluorescence, Staining, Expressing, Western Blot

Journal: The Journal of Physiology

Article Title: Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5

doi: 10.1113/jphysiol.2012.240952

Figure Lengend Snippet: Bivariate correlation analysis

Article Snippet: The specificity of the PLIN5 antibody was confirmed in a competition experiment in which a PLIN5 recombinant peptide (Cat. no. NB110–60511PEP; Novus Biologicals, Cambridge, UK) was incubated with the PLIN5 primary antibody, subsequently resulting in removal of the positive fluorescent signal for PLIN5 (Supplementary Fig. S1 ).

Techniques: